Andreas Teske

Cruise Planning Questionnaire

Ship:	ATLANTIS
Vehicle(s) required:	ALVIN
Name of ship for ROV ops:

Ch Sci:	Andreas Teske
Affiliation:	University of North Carolina at Chapel Hill
Address:	Dept of Marine Sciences, 340 Chapman Hall, CB 3300 27599 Chapel Hill
Phone:	919-843-2463
Email:	teske@email.unc.edu
POC / contact info:

Departure port & date:	Guaymas, Nov 22
Distance to first station:	ca. 100 miles
Number of stations:	1
Distance between stations:	0
Total days on station:	15
Arrival port & date:	Guaymas, Dec 6

Op area:	NP 13
Lat/Lon:	27°N00.30 to 27°N00.60 and 111°W24.65 to 111°W24.35
Depth range:	2000

Will the vessel be operating within 200 NM of a foreign country?
Which country?
Are visas or special travel documents required?

Funding agency:

NSF

	Specify:

Grant or contract number: 0647633

Science objectives/activities planned:

Our Science objectives focus on A) how the supply of geothermally derived substrates determines the composition, spatial zonation, and in-situ metabolic rates of the active microbial community, and B) how diverse cultured and uncultured microorganisms participate in carbon assimilation and transformation pathways. In the Guaymas Basin sediments, physiologically distinct microbial communities channel the geothermally produced substrates in hydrothermal fluid that permeates the sediments through a double “microbial gauntlet” of anaerobic metabolism and chemolithoautotrophic C fixation.
For combined geochemical/microbiological analysis, we are planning extensive push core sampling of hydrothermally and micreobially active sediments; Beggiatoa mat sampling using push cores and slurp guns, and also some bottom water sampling adjacent to microbially active sediments and microbial mats. Prior to sediment coring, benthic processes and microgradients within the sediments will be determined with redox-profiler modules, benthic respirometry chambers, and in-situ sulfate-reduction injectors and incubators. In-situ temperature gradients will be measured by Alvin thermoprobe and newly build UNC temperature loggers, to identify sediments with steep temperature (and, by inference, geochemical and microbiological) gradients. These sediments will be preferentially chosen for our work. As a working hypothesis, Beggiatoa mats may to some extent represent visual proxies for geochemically and microbiologically active sediments.

As part of the pre-cruise planning procedure, would you prefer (check one or both) a teleconference

or a visit to WHOI

WHOI shipboard scientific instrumentation desired:

12 kHz bathymetry system
		Fume hood
Relay transponder for wire use		Refrigerator
Piston corer	Deionized water system
	CTD/rosette system	-70 deg freezer
Box corer		Walk-in cooler (AT only)
	10-liter Niskin bottles
	Pinger for wire use

Is nighttime work anticipated on this cruise? No

Critical MET sensors:
Critical CTD sensors:	Temperature, salinity, density, oxygen, fluorescence, transmission

Do you need Seabeam? (AT and KN only) No

Long baseline (LBL) transponder navigation? Yes No. of xpndrs/nets: 3 0r 4
Ultra-short baseline (USBL) navigation? No
Doppler/GPS navigation? No

Will you be using portable science vans? Yes How many: 1

	Science Van #1	Science Van #2	Science Van #3
Type/size	Radioisotope van
Location
Water	yes
Power	yes

Anticipated standard oceanographic cable use:

	Instruments(s)	Instrument Weight(s)	Maximum Depth
Conducting hydro
Steel hydro
Steel trawl
.681" fiber optic

Do you require slip rings?			Number of conductors:
Do you need non-standard wire?			If so, what type?
Do you need a traction winch?

Will you be bringing any equipment (winches, blocks, etc.) that lowers equipment over the side? No

If yes, please provide details:
Will there be special requirements for:

Electrical power	Yes
Equipment handling
Inter/intraship communications
Science stowage

If yes to any of the above, please identify: Some European lab gear might require 220V, but everything worked well the last time

Will any of the following items be used on board?

Explosive devices		No
Portable air compressors		No
Flammable gases		No
Radioisotopes	Yes
Isotope van	Yes

Will hazardous material be used aboard, or deployed by an ROV? Yes

What measures or plans have been made a) in the event of a spill, and b) for the removal and/or disposal of these materials at the completion of the cruise?
Hazardous material will be accompanied by the respective spill kit.
We plan to take chemicals, waste and gases off the ship at the next port. The German container (which includes the hazardous waste and radioisotopes) is offloaded in Manzanillo, Dec 12-15.

Vehicle equipment required:

Check one or both: ALVIN

Major water samplers
Large capacity slurp samplers	Yes		Multi-chamber
Small capacity slurp samplers	Yes
Bio collection boxes	Yes		12" x 12" x 24"
Push corers	Yes
Scoop nets	Yes
High temperature probe	Yes
Low temperature probe	Yes
Heat flow probe	Yes
Magnetometer
Portable CTD	Yes
Digital Still Camera	Yes		Down-looking
Search Sonar
Profiling Sonar		(ALVIN only)
Rock Drill *(Jason only*)
Video Duplication *(Jason)*
Elevators	Yes

Other vehicle-related equipment (list):	No. 1: Profiler-Module No. 2: Benthic Chamber No. 3: INSINC injection system

Science equipment to be installed on vehicle:	The following instruments will be lowered using elevators; they will not be tethered to cables. No. 1: Profiler-Module The Profiler-Module is an autonomous system carrying up to 11 different electrochemical sensors (e.g. O2, pH, H2S, T), which can be designed (e.g. sensor length and tip diameter) prior to its applications. The module will be placed at targeted sites to measure high-resolution gradients across the sediment-water interface. The Profiler works completely autonomous and during the deployment the program is controlled from the ship via the ROV. Pressing a switch at the Profiler frame with the ROV-manipulator activates the measuring routine (one profiling cycle takes 2-3 hr) allowing several cycles to be performed during one dive. Between each measuring cycle the ROV will place the Profiler at different spots. Ideally, the profiler has to be carried with the Lift/Shuttle-System to the seafloor. Due to the small size it might also be possible to transport it directly on the porch of the ROV. Technical description Dimension: 950 x 700 x 650 mm Weight in air 78 kg in water 25 kg Battery capacity 30 hours No. 2: Benthic Chamber The chamber-module is an autonomous system equipped with a circular chamber (diameter 19 cm) enclosing a sediment area of 283 cm 2 with a 10-15 cm overlying water column. Two Clark-type mini-electrodes record the decrease (<25%) of oxygen inside the chamber over time. Up to 10 pre-programmed water samples can be taken from the chamber during the incubation for analysis of DIC, nutrients, and other dissolved components (e.g. sulfide, methane). One chamber incubation last 6-8 hours allowing several incubations to done during one ROV dive. Ideally, the benthic chamber has to be carried with the Lift/shuttle-System to the seafloor. Due to the small size it might also be possible to transport it directly on the porch of the ROV. Technical description (present configuration) Dimension: 800 x 950 x 680 mm Weight in air 70 kg, in water 25 kg Battery capacity 30 hours No. 3: INSINC injection system The tracer injection system INSINC works like a push core (Fig 3). The core liner is pushed into the sediment by the ROV manipulator. After taking the sediment sample the core is placed in a special sheath. When placed in the sheath the injection mechanism is released by pulling on a robe at the head of the INSINC core. Injecting the tracer while the core is placed in the sheath guarantees that no tracer leaks into the environment. The incubation of the sediment sample lasts for 6-24 hour during which the core has to stay at the seafloor. For one measurement a set of 4 INSINC corewill be taken The INSIC cores can be carried as the standard push cores; a. in the basket of the ROV or b. as module in the Lift/Shuttle-System. Technical description (present configuration) for single INSINC core Dimension: length 420 mm; OD 100 mm Weight in air 4.5 kg; in water 2.6 kg (ca. 6 kg with sediment)

Electronic equipment used in the ALVIN personnel sphere:	1 laptop, 1 digital camera

Other comments and information:	The benthic chamber (Equipment Item 2) might not be available; most likely we are working only with the in-situ profiler and INSINC cores.

Date Submitted:

07/22/2009 01:50 PM